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We used Vesi-Ref CD63-GFP to validate our Light-induced Extracellular Vesicle Adsorption (LEVA) technique, which rapidly and selectively modifies the chemistry of surfaces at the microscale, allowing the precise adsorption of EVs from various sources. Vesi-Ref CD63-GFP were patterned onto coverslips using different templates including both circular and microtrack-shaped resolution tests, as well as different gradient patterns (Gaussian, exponential, linear). Vesi-Ref CD63-GFP were imaged using total internal reflective fluorescence (TIRF) microscopy (Nikon), allowing clear visualization of EVs patterned in the desired regions, with minimal adsorption in the undesired regions. Vesi-Ref CD63-GFP complemented our use of dye-only controls in validating the LEVA technique, and in combination with other results, encouraged us to move forward with biomedical applications described in our recent manuscript

Colin Hisey

Post Doctoral Scholar, Biomedical, Chemical and Biomolecular Engineering

Dr. Colin Hisey earned B.Eng. and M.S. degrees in Chemical Engineering from the University of Dayton and a Ph.D. in Biomedical Engineering from The Ohio State University in 2018.

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