Publications

Richard J.R. Kelwick, Alexander J. Webb, Amelie Heliot, Paul S. Freemont

https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(26)00190-3

Haiyan An, Ceri Battle, Megan Chambers, Nicholas Jones, Tyler J. Joseph, Iain Perry, Molly E. Raikes, Sophie G. Reed, April Rees, Oliver Richards, Catherine A. Thornton, Hannah Toghill, Jason Webber, Luke Newey, Hui Zhang.

https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.70288

Benjamin T. Vyzourek, Dirk Anderson, Luke Skrabal, Christine E. Humphrey, Eduardo Romero, Brittany Schweiger, Forrest Kievit, Jeremy R. Miles, Angela K. Pannier

https://www.sciencedirect.com/science/article/pii/S2773041725000368?via%3Dihub

Raquel Mesquita-Ribeiro, Alex Rathbone, Paula Jackson, Sophie McCann, Hannah Jackson, Cristiano Lucci, Victoria James, Federico Dajas-Bailador

https://www.biorxiv.org/content/10.1101/2025.10.24.684188v1.full

Colin Hisey, Xilal Rima, Jacob Doon-Ralls, Chiranth Nagaraj, Sophia Mayone, Kim Truc Nguyen, Sydney Wiggins, Kalpana Deepa Priya Dorayappan, Xin Huang, Mangesh Hade, Karuppaiyah Selvendiran, James Higginbotham, Oleg Tutanov, Jeffrey Franklin, Robert Coffey, David Wood, Chunyu Hu, Divya Patel, Setty Magaña, Andre Palmer, Derek Hansford, Eduardo Reátegui

https://www.nature.com/articles/s41592-025-02914-w

Svetlana Pavlova, Doste R. Mamand, Daniel W. Hagey, Xiuming Liang, H. Yesid Estupiñán, Wenyi Zheng, Guannan Zhou, Risul Amin, Antje M. Zickler, Scott Bonner-Harris, Zankruti Dave, Miina Ojansivu, Manuela O. Gustafsson, Oliver G. Hayes, Oscar P.B. Wiklander, Manuchehr Abedi‐Valugerdi, Samantha Roudi, Antonin Marquant, Joel Z. Nordin, Molly M. Stevens, Samir EL Andaloussi

https://www.sciencedirect.com/science/article/pii/S2773041725000411?via%3Dihub

André Görgens & Samir El Andaloussi

https://link.springer.com/protocol/10.1007/978-1-0716-4905-3_10

• Cell-free extracellular vesicle (EV) engineering could be utilised to rapidly prototype and test novel biotechnological, vaccine, or therapeutic EVs for foundational or translational applications.
• Our data highlight some of the impacts that hollow fibre-based cell culture, EV isolation methods and different cell-free engineering approaches have on cell-free EV engineering workflows.
• We demonstrate several characterisation assays and technologies, including nanoflow cytometry, that can be used to assess cell-free EV engineering efficiency.

Richard J. R. Kelwick, Alexander J. Webb, Amelie Heliot, Paul S. Freemont

https://www.biorxiv.org/content/10.1101/2025.09.20.677537v1.full
https://doi.org/10.1101/2025.09.20.677537

Tomoharu Tanaka, Satoru Morimoto, Keitaro Ito, Kaori Yasutake, Chris Kato, Munehisa Shinozaki, Kota Suda, Takeshi Maeda, Yoshiyuki Yato, Masaya Nakamura, Hideyuki Okano & Narihito Nagoshi

https://www.nature.com/articles/s42003-025-08947-3

Minimal information for studies of extracellular vesicles(MISEV2023): From basic to advanced approaches

by Bigboy Simbi 1, Ryan C. Pink 2, Louise Whatford 3ORCID and Charlotte Lawson 1,*

1. Comparative Biomedical Sciences, Royal Veterinary College, London NW1 0TU, UK
2. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK
3. Pathobiology and Population Sciences, Royal Veterinary College, Hertfordshire AL9 7TA, UK

* Author to whom correspondence should be addressed.
Dairy 2024, 5(1), 161-172; https://doi.org/10.3390/dairy5010013
Submission received: 30 December 2023 / Revised: 27 January 2024 / Accepted: 5 February 2024 / Published: 8 February 2024

https://www.mdpi.com/2624-862X/5/1/13

Minimal information for studies of extracellular vesicles(MISEV2023): From basic to advanced approaches

Joshua A. Welsh, Deborah C. I. Goberdhan, Lorraine O’Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A. P. Driedonks, Uta Erdbrügger, Juan M. Falcon-Perez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Federico Colombo, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic-Talisman, Nicole A. Kruh-Garcia, Vroniqa Ku’ulei-Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens-Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter-Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin Pol, Martijn J. C. Herwijnen, Marca H. M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, MISEV Consortium, Clotilde Théry, Kenneth W. Witwer

https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jev2.12404
https://doi.org/10.1002/jev2.12404

Standardization of extracellular vesicle concentration measurements by flow cytometry: the past, present, and future

Britta A. Bettin, Zoltán Varga, Rienk Nieuwland and Edwin van der Pol

JThrombHaemost.2023;▪:1–13

https://doi.org/10.1016/j.jtha.2023.04.042

https://www.jthjournal.org/article/S1538-7836(23)00413-0/fulltext

Review of structural design guiding the development of lipid nanoparticles for nucleic acid delivery

Marité Cárdenas, Richard A. Campbell, Marianna Yanez Arteta, M. Jayne Lawrence & Federica Sebastiani, Current Opinion in Colloid & Interface Science, 2023, 101705, ISSN 1359-0294, DOI: 10.1016/j.cocis.2023.101705.

(https://www.sciencedirect.com/science/article/pii/S1359029423000304?via%3Dihub)

Analytical methods for process and product characterization of recombinant adeno-associated virus-based gene therapies,

Andreas L. Gimpel, Georgios Katsikis, Sha Sha, Andrew John Maloney, Moo Sun Hong, Tam N.T. Nguyen, Jacqueline Wolfrum, Stacy L. Springs, Anthony J. Sinskey, Scott R. Manalis, Paul W. Barone & Richard D. Braatz, Molecular Therapy – Methods & Clinical Development, Volume 20, 2021, Pages 740-754, ISSN 2329-0501, DOI: 10.1016/j.omtm.2021.02.010.

(https://www.sciencedirect.com/science/article/pii/S2329050121000255)