Fluorescence staining/labelling of extracellular vesicles, viruses and other nanoparticles is the most commonly used method to detect and track them during the cellular uptake process.
Various methods utilising the fluorescence emission of the tracer molecule, such as fluorescence spectroscopy, microscopy, and flow cytometry, are employed for analysis. Among these, flow cytometry stands out with its advantages of speed, high sensitivity, and the ability to statistically analyse multiparametric data. Furthermore, it facilitates the simultaneous detection of various cell properties, including cell size, viability, intracellular pH, and membrane potential. Flow cytometry has also been commonly utilised for monitoring endocytosis.
Vesi-Dye LMB probes combine specificity, brightness and long-term stability, essential qualities to perform cell uptake assays. Combined with Vesi-SEC micro clean-up spin columns, it offers a stable, contaminant-free labelled sample that will truly reflect the potential for the studied entities to be taken up by target cells.