Skip to main content

Vesi-Dye LMB-600

Vesiculab

Vesi-Dye LMB-600: Water-soluble fluorescent probes specific to lipidic bi-layer membranes.

The Vesi-Dye LMB family of fluorescent probes bring reliability and predictability to functional bio-nanoparticle analysis.

ORDER ONLINE HERE
Vesi-Dye LMB-600: Water-soluble fluorescent probes specific to lipidic bi-layer membranes.
Vesi-Dye LMB-600: Water-soluble fluorescent probes specific to lipidic bi-layer membranes.

Vesi-dye fluorescent probes solve these issues with a chemical composition that delivers:

  • Specificity: labelling only lipidic bi-layers, rather than with any hydrophobic moiety
  • Compatibility: water-solubility makes Vesi-dye probes easy to manipulate in aqueous media
  • Reliability: no micelles or aggregates are formed during the incubation process
  • Performance: very bright and stable for 24 hours, Vesi-dye-labelled samples won’t quench prematurely
request a quotation
Vesi-Dye LMB-600: Water-soluble fluorescent probes specific to lipidic bi-layer membranes.
Vesi-Dye LMB-600

Enabling flow cytometric analysis at the nanoscale

In flow cytometry, measuring nanoparticles by means of scatter detection isn’t always possible as its intensity isn’t strong enough to emerge from the background signal. This has kept entities such as extracellular vesicles, (EVs, exosomes, etc. ), viral vectors for gene therapies (lentiviruses, etc.) and nanoparticles-bases drug carriers (liposomes, LNPs, etc.) out of reach for even the most sensitive flow cytometer. Labelling these entities with lipophilic fluorescent probes has allowed an enhancement of the signal strength by switching from scatter to fluorescent, but the probes themselves were found to be unspecific, poorly soluble in aqueous buffers or producing nano-sized micelles or aggregates.

ORDER ONLINE HERE
Vesi-Dye LMB-600

Controlling for impurities in F-NTA and F-MRPS

Second-generations nanoparticle tracking analysis (NTA) and microfluidics resistive pulse sensing (MRPS) allow fluorescence detection, which can be used to complement light scatter data to detect non-sample events.

One common occurrence in NTA is the presence of nano-sized bubbles and solid mineral impurities contained in the dilution buffer. These substrates generate events that are included in the final data report, potentially compromising the accuracy and reliability of sample concentration measurement.

Equally in MRPS, pulse sensing detection only cannot ascertain the nature of all particles measured in the size range of interest.

Vesi-dye can be specifically applied to the extracellular vesicles, lentivirus or liposome sample to reveal the presence of the lipid bi-layer by fluorescence analysis. Under these conditions, only the fluorescence-positives events will be counted for the size and concentration report.

Vesi-Dye LMB-600 Technical Description

Excitation488-525mm
Emission600-640mm
SolubleWater Soluble
Stability24 hours after incubation
Format100 applications
request technical information
en_GBEnglish