Vesi-Ref CD63-GFP

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Vesi-Ref CD63-GFP: Fluorescent extracellular vesicle standard for nano-bio research

Assess → Control → Progress
Vesi-Ref CD63-GFP introduces certainly and repeatability in functional nanoparticles analysis. By assessing the limits of detection of analytical instruments used to measure size, concentration and biomarkers by fluorescence labelling, it is possible to confirm that unknown samples are correctly characterised for these properties.

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Description

Vesi-Ref CD63-GFP: Fluorescent extracellular vesicle standard for nano-bio research

Assess → Control → Progress
Vesi-Ref CD63-GFP introduces certainly and repeatability in functional nanoparticles analysis. By assessing the limits of detection of analytical instruments used to measure size, concentration and biomarkers by fluorescence labelling, it is possible to confirm that unknown samples are correctly characterised for these properties.

Size and concentration measurement:
Extracellular vesicles are characteristically broad in size distribution, making the assessment of their size potentially difficult with most analytical methods. Before embarking on a long session, run an aliquot of Vesi-Ref CD63-GFP to check the output. Adjust acquisition parameters if size distribution is truncated/not in nominal range or if particle concentration is over/under counted

Applicable to:

  • Nanoparticle Analysis (NTA)
  • Dynamic Light Scattering (DLS)
  • Resistive Pulse Sensing (TRPS and mRPS)
  • Transmission Electron Microscopy (TEM)
  • Single Particle Interferometric Reflectance Image Sensing (SP-IRIS)
  • Nano-Flow Cytometry (nFCM)

Phenotyping by fluorescent labelling:
Assessing the sensitivity of detection in fluorescence is crucial, as under or over representation of signal can lead to unsuccessful experiments. Vesi-Ref CD63 GFP’s strong GFP signal enables to control the sensitivity of fluorescence detection in most modern techniques aimed at bio-nanoparticles analysis, including:

  • Flow Cytometry (cell analyzers and FACS sorters)
  • Fluorescence Nanoparticle Tracking Analysis (f-NTA)
  • Single Particle Interferometric Reflectance Image Sensing (SP-IRIS)
  • Nano-Flow Cytometry (nFCM)

Technical Description

OriginHuman embryonic kidney cells (HEK293)
Quantity100µL (1×109 particles)
Storage conditions (lyophilised)Up to 1 years at 4°C
Storage (reconstituted suspension)Up to 6 months at -80°C. Up to one month at -20°C.